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:: Volume 22, Number 1 (10-2016) ::
3 2016, 22(1): 41-46 Back to browse issues page
Occult Hepatitis B Virus Infection among Cornea Donors in the Central Eye Bank of Iran
S Samiee, M Rezaei Kanavi , MA Javadi, A Bagheri, K Bayat Makoo
Abstract:   (472 Views)

Purpose: This study was designed to detect occult hepatitis B virus (HBV) infection among cornea donors to the Central Eye Bank of Iran using polymerase chain reaction (PCR).

Methods: Cornea donors with negative screening serologic results for HBS Ag, HBS Ab, HCV Ab, HIV Ab, HTLV Ab, and syphilis, and positive serology for HB Core Ab, underwent real-time PCR to detect HBV DNA with a detection level of 400IU/mL.

Results: Over a 2-year period, 8372 sera from 8372 cornea donors were enrolled; 76 (0.9%) out of which were serology negative for abovementioned antibodies except for HB Core Ab. Real-time PCR performed in these 76 cases detected HBV DNA in 6 (7.9%) cases. The sera were inadequate for PCR test in 4 cases (5.3%). HBV DNA was not detectable in 66 cases (86.8%), and hence the corresponding corneas were released for transplantation.

Conclusion: Nucleic acid amplification test for HBV is necessitated for detection of occult HBV in cornea donors in order to minimize the risk of HBV transmission via corneal transplantation. In the present study, HBV DNA was undetectable in the majority of cases who were suspicious of occult HBV infection, leading to release of the corresponding corneas for transplantation. These tissues used to be discarded because of inability to evaluate HBA DNA.

Keywords: Cornea Donors, Eye Bank, Hepatitis B virus, Occult HBV, Real Time PCR
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Type of Study: Research | Subject: Ophthalmology
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Samiee S, Rezaei Kanavi M, Javadi M, Bagheri A, Bayat Makoo K. Occult Hepatitis B Virus Infection among Cornea Donors in the Central Eye Bank of Iran. 3. 2016; 22 (1) :41-46
URL: http://binajournal.org/article-1-847-en.html
Volume 22, Number 1 (10-2016) Back to browse issues page
مجله چشم‌پزشکی بینا Bina Journal of Ophthalmology
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