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:: Volume 15, Issue 3 (4-2010) ::
3 2010, 15(3): 213-217 Back to browse issues page
Confocal and Transmission Electron Microscopic Features of Endothelial Vacuolation in Donated Corneas
M Rezaei Kanavi MD , MA Javadi MD, T Chamani
Shahid Beheshti University of Medical Sciences
Abstract:   (2512 Views)

 

Purpose: To report the confocal microscopic features of endothelial vacuolation in donated corneas and to confirm the observed changes by transmission electron microscopy.

Methods: Confocal and transmission electron microscopy were performed on donated corneas with endothelial vacuole formation on slitlamp biomicroscopy. The ultrastructural findings were also compared with those of a normal-looking endothelium from a keratoconic cornea.

Results: Confocal microscopy revealed large numbers of dark round to oval-shaped structures within the endothelium, consistent with vacuolation which was confirmed by light and electron microscopy by the presence of variable-sized cyst-like structures within the endothelial cytoplasm mainly near the posterior cytoplasmic membrane in semithin preparations and observation of electron-lucent and relatively large-sized intracytoplasmic vacuoles on transmission electron microscopy. These features were not observed in the normal-looking endothelium of a keratoconic cornea.

Conclusion: In this report, confocal microscopic features of endothelial cell vacuolation are presented in donated corneas and confirmed by transmission electron microscopy. The confocal microscopic images can serve as a useful reference image for endothelial vacuolation.

Keywords: Confocal, Cornea, Endothelium, Vacuole
Full-Text [PDF 311 kb]   (420 Downloads)    
Type of Study: Research | Subject: Ophthalmology
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Rezaei Kanavi M, Javadi M, Chamani T. Confocal and Transmission Electron Microscopic Features of Endothelial Vacuolation in Donated Corneas. 3. 2010; 15 (3) :213-217
URL: http://binajournal.org/article-1-337-en.html


Volume 15, Issue 3 (4-2010) Back to browse issues page
مجله چشم‌پزشکی بینا Bina Journal of Ophthalmology
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